Pdf the antioxidant potential of some herbal plant extracts commercial products was measured using various in vitro assays. The antioxidant activity of the two plant crude extracts against 2,2diphenyl1picrylhydrazyl dpph radical was determined by uv spectrophotometry at 518 nm. Antioxidant activity, total phenolic and total flavonoid. The leaves extracts show a higher activity on cat and asa peroxidase activity, while the. The many number of medicinal plants are used in the cellular and metabolic. Pdf evaluation of antioxidant activity of some plant. Phytochemical study and evaluation of the antioxidant.
In vitro nitric oxide scavenging activity of methanol. Among the important biological properties exhibited by plant polyphenols, their antioxidant activity has raised a great interest 1. Antibacterial, antioxidant activity of ethanolic plant. Antioxidant activity of polyphenolic plant extracts. Herbal plants considered as good antioxidant since ancient times. Antioxidant activity of methanol extracts of different parts of lantana. Green tea, oligomeric procyanidins from grape seed and pine bark, bilberry, and ginkgo exhibited taa in the range of 5. In the present paper we have investigated antioxidant activity of extracts from withania somnifera dunal. The results indicated that both leaves and seed extracts have an antioxidant activity. Evaluation of antioxidant capacity of plant extracts by. In sum, the antioxidant activities of ethanolic extracts from m. However, the total antioxidant activities of plant extracts cannot be evaluated by using one single method, due to the complex composition of phytochemicals as well as of oxidative processes. The present study evaluated the antioxidant effects of methanolic seed extract of a. Ethiopia is one of the developing countries which have enormous diversity of plants.
In vitro antioxidant activity of extracts from the leaves of felicia. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase. Fruit extracts for total phenolics and antioxidant activity measured in methanol extract aoam analysis were prepared using the method of swain and hillis 1959, with some modi. Preliminary phytochemical screening, quantitative analysis. Evaluation of antioxidant activity of some plant extracts. Methanolic extracts of cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. Hence, in this study prepawe red polyphenol extracts from papaya peel pp with the aim of exploiting potential antioxidant and anticancer properties of pp extracts. Comparative analysis of the antioxidant activity of cassia. The antioxidant activity and phenolic contents of the stem as determined by the dpph, proanthocyanidins, total.
Screening of antioxidant activity and phenolic content of 24. Screening of in vitro antioxidant activity of methanolic. Effect of extraction solventtechnique on the antioxidant activity of selected medicinal plant extracts article pdf available in molecules 146. There are many methods of evaluating the antioxidant activity of plant extracts, and in this study we have chosen to evaluate the antioxidant activity of plectranthus aromaticus extracts using two different methods. Antioxidant activity in extracts from coriander sciencedirect. Three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl1 picrylhydrazyl dpph radical scavenging method, static. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of anabasis aphylla l. Determination of antioxidant activity the antioxidant activity of plant extracts against peroxidation of linoleic acid was determined by the reported method 16. Evaluation of antioxidant activity of medicinal plant extracts. The content of total phenolics in the extracts was determined spectrometrically according to the folin. Research article open access antioxidant activity, total. Extract, free radical, antioxidant activity, medicinal plant. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan. The ethanolic extracts exhibited moderate antimicrobial activities, whereas the aqueous extracts showed poor antimicrobial properties in.
This result identifies this plant as a good candidate for further investigations. Pdf methods for determining the antioxidant activity. Different concentrations of the plant extracts were prepared using analytical methanol 1, 3, 7, 10, 20, 30, 40. Screening of brazilian plant extracts for antioxidant. Antioxidant activity of polyphenolic plant extracts mdpi. Preparation of plant extracts for antioxidant evaluation about 10 g of the grounded plant were soaked in 1 liter of methanol 99% and put in a shaker device at 100 rounds per minute for 72 hours at room temperature and stored in refrigerator for 4 days. Commonly used medicinal plant extracts with standardized content of polyphenols were investigated for their total antioxidant activity taa. Antioxidant activity of various tea extracts in relation to their antimutagenicity gowchin yen, and huiyin chen j. The significant linear correlation was confirmed between the values for the total phenolic content and antioxidant activity of plant extracts. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig. Four complementary methods were used to determine the antioxidant activity of methanolic extracts. Antioxidant properties of several medicinal plants growing.
A detailed study was performed on the antioxidant activity of the methanol extract of whole. Antioxidant properties of the methanol extracts of the leaves. Antioxidant and apoptotic activity of papaya peel extracts in. If plant extracts contain antioxidant activity, it may clearup free radicals from the body, and boost the immune system to handle the infection or illness effectively. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method.
The activity was measured according to the method previously described. Gc and carotene bleaching test bcbt, have been compared with regard to their application in the screening of plant extracts. Antioxidant properties of plant extracts edelweiss publications. Extraction and determination of antioxidant activity of. Materials and methods fresh mature papaya fruits variety caribbean red were obtained from a local health food store, huntsville, al. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase. In the previous chapters 3 and 4, it was shown that p. Antioxidant activity of various tea extracts in relation to. Antioxidant activity of plant extracts and their effect on. Antioxidant activity of some common medicinal plants. A comparative study on the antioxidant activity of methanolic. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order.
Preliminary phytochemical screening, quantitative analysis of. The free radical scavenging action of methanol extracts of plant are in the order as desmodium gangeticum amaranthus caudatus solanum nigrum. The scavenging activity of the plant extract through the annihilation of the dpph radicals was investigated. Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. Pdf antioxidant activity of polyphenolic plant extracts. Pdf evaluation of antioxidant activity of medicinal plant extracts. Dpph free radical scavenging activity of the extracts of. Witch hazel, propolis epid, artichoke, and hawthorn afforded. Antimicrobial and antioxidant activity of essential oil. Comparatively, methanolic extract showed higher activity than ethanolic activity. Phytochemical screening and antioxidant activity of ethanol extract. Tocopherol was used as a standard to evaluate the antioxidative activity of samples. The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75c.
Original article comparison of abts, dpph, frap, and orac. The reacted solution obtained 1 ml was used for tba assay. Jan 14, 2002 three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl. Evaluation of antioxidant activity of some plant extracts and their application in biscuits. Numerous works devoted to antioxidant activity of plant extracts are published. Preliminary phytochemical screening, quantitative estimation. The effect of various plant extracts was measured on several important oxidation indicators. Antioxidant activity determination of citronellal and. Assessment of antioxidant activity of plant extracts by. Frap, dpph and total phenols by the folincitocalteau electron transfer methods and abts a method. Antioxidant activity of polyphenolic plant extracts by dimitrios stagos department of biochemistry and biotechnology, university of thessaly, 41500 biopolis, greece.
Three grams of guava tissue were mixed with 25ml methanol and homogenized using the ultraturrax homogenizer. Evaluation of the in vitro and in vivo antioxidant. Pdf in vitro antioxidant and antiproliferative activity of. Evaluation of antioxidant activity of some plant extracts and. In these works aoa are given in units % mg, mgml or mgg, that character. Phytochemical screening and evaluation of antioxidant and. Evaluation of antioxidant activity of medicinal plant 885 figure 2. The antioxidant activities of the methanol extracts from the leaves and stems of celtis africana ulmaceae were assessed in an effort to validate the medicinal potential of the subterranean part of the herb. The antioxidant activities were determined by total antioxidant capacity, dpph 1,1diphenyl2picrylhydrazine radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal plants rozina parul1, sukalayan kumar kundu 2 and pijush saha2 1. Leaf disc assays for rapid measurement of antioxidant activity.
Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. Ferric reducing antioxidant potential assay frap assay the frap assay was employed to estimate the antioxidant capacity of the samples in vitro. Aug 19, 2015 the antioxidant activity of plant extracts is not limited to phenolics javanmardi et al. Screening of plant extracts for antioxidant activity. Antioxidant and potential antiinflammatory activity of. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. A comparative study on the antioxidant activity of. Felicia muricata is a medicinal plant used for the management of different human and livestock diseases in the eastern cape province of south. For a typical assay an aliquot of 20 l of three dilutions of each extract in ethanol 0. A report on the in vitro antioxidant properties of vachellia karroo.
The extracts were investigated for the antioxidant activity using 2, 2 diphenyl, 1 picryl hydrazyl. Antioxidant activity of plant extracts containing phenolic. To observe the antioxidant activity of leaves and seed extracts of n. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal. Inhibition of microsomal peroxidation data represents, mean sem, for n3 the reducing power of different commercial extracts was performed and showed. The concentration of extracts in lard varied from 0. Antioxidant activity determination of citronellal and crude. Screening of medicinal plant extracts for antioxidant activity. Antioxidant activity of polyphenolic plant extracts dimitrios stagos department of biochemistry and biotechnology, university of thessaly, 41500 biopolis, greece.
Antioxidant activity, active nitrogen species, sodium nitroprusside. Antioxidant properties of extracts from selected plant materials. Screening of plant extracts for antioxidant activity wiley online. From this study it is observed that all the plants posses marked antioxidant effect. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts torilis leptophylla l naima saeed, muhammad r khan and maria shabbir abstract background. Antioxidant activity % control dr dr sample dr control 100. Pdf in vitro antioxidant and antiproliferative activity. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to. Antioxidant properties of the methanol extracts of the. Consequently, the search and study of plant extracts that have the highest antiradical and antioxidant activity aoa is a very urgent task. The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant activity. In vitro antioxidant and antimicrobial activity of.
The present study reveals that extracts are a good source of antioxidant property containg phytoconstituents. Researcharticle preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan. Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Furthermore, it was estimated that more than 50% of prescribed drugs are either directly derived from herbsplants or active ingredients obtained from plant. The total free radical quenching dpph assay revealed the antioxidant activity of ic 50 9.
In the present study, three plant foods, namely, drumstick leaves moringa oleifera, mint leaves mentha spicata and carrot tuber daucus carota were extracted with ethanol and analyzed for their antioxidant activity. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Total antioxidant activity and phenols and flavonoids content of several plant extracts claudiavalentina popa1, liliana lungu 2, manuela savoiu, corina bradu1, vasile dinoiu2, and andrei florin danet1 1faculty of chemistry, university of bucharest, bucharest, romania 2institute of organic chemistry c. Dpph free radical scavenging activity of the extracts of the. The antioxidant activity of the plant extracts was examined on the basis of the scavenging effect on the stable dpph free radical activity braca et al. Antioxidant and antilipid peroxidation activities of leaves. Antioxidant activity of some plant extracts of the family. The presence of flavanoids,tannins,steroids and saponins present in extracts may be responsible for antioxidant activity. Evaluation of antioxidant activity of medicinal plant. Abstract identification and characterization of natural products with antioxidant and antiproliferative activity has received much interest over the past few years. Pdf different extracts from five medicinal plants in terms of phenolic compounds and antioxidant properties were studied. Among the extracts, tsb showed the highest antioxidant activity followed by trb, tf. The antioxidant activity of extracts was evaluated according to the amount of malonaldehyde mda formed by the feso 4induced oxidation of linoleic acid and a high pufa oil.
Phytochemical screening and antioxidant activity of moroccan. Pdf screening of medicinal plant extracts for antioxidant. Department of pharmacy, gono bishwabidyalay, savar, dhaka 44, bangladesh. Figure 1 shows the amount of each extract required for 50% inhibition of dpph activity ic 50. The antioxidant activity may also contribute from the other antioxidant secondary metabolites such as volatile oils, carotenoids and vitamins wongsa et al. Antioxidant properties of the methanol extracts of the leaves and stems of celtis africana. Mar 16, 2001 screening of brazilian plant extracts for antioxidant activity by the use of dpph free radical method luciana l. Estimation of the total flavonoids in the plant extracts was carried out.